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Here, we pharmacologically targeted the mitoribosome to induce aberrant mitochondrial translation (mistranslation) resulting in m ablation and inhibit mitochondrial translation (nontranslation) resulting in subsequent loss of OXPHOS complexes, in order to identify pathways increasing cell fitness

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By blocking NNMT, 5-Amino-1MQ prevents nicotinamide methylation, allowing NAD+ levels to rise and methyl groups to remain available for histone methylation and other epigenetic processes

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This prompted Marks to devise a simpler method