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10.1016/j.pcd.2015.10.009 41 FlemmingN

We rely on NAD+ for our well-being because its involved in more than 500 enzymatic reactions in the body, may help repair DNA, and it converts food into the energy our cells need to thrive

When colonies were obviously formed, 4% paraformaldehyde (PFA) was added to each well for 30 min at 4 C, followed by crystal violet solution for cell staining for at least 30 min