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In vivo juxtacellular recordings of VTA DA neurons Mice were deeply anaesthetized with chloral hydrate (8%), 400 mg/kg IP, supplemented as required to maintain optimal anesthesia throughout the experiment

(15) Other theories for biological mechanism linking smoking to TED have also been proposed: smoking may have a direct irritant action on the eye, causing inflammatory changes, smoking may be a cause of stimulation of the autoimmune process in smokers and smoking may be influence the secretion of cytokine in the eye

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