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DNA expression plasmids were extracted and were purified from bacterial cells with NucleoBond Extra Midi Plus kit (Macherey-Nagel, Germany) and they were stored at -20C until their use for transient transfection into mammalian cells

For the purpose of this study, 10 to 12 week old inbred male Lewis rats weighing approximately 300 grams each were used

The first paper to show that prefibrillar, aggregated forms of non-disease-associated proteins are highly toxic to cells

They are not intended for human consumption, medical, diagnostic, or therapeutic applications

Men who reached their first dose escalation may experience temporary symptom recurrence that then resolves