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Culture conditions 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Hams Nutrient Mixture F12 (Merck, 51651 C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20 ng/ml EGF (Peprotech, AF-100-15), 10 ng/ml FGF-basic (Peprotech, 100-18B), and 10 ng/ml PDGF-AA (PeproTech, 100-13 A)

Translational Research

I think the most common one I've seen in my patients is acupuncture

Injection of mice with heparinase I/III can reduce lung compliance, increase MMP-7 and MMP-9 expression, and cleave HS in the glycocalyx [70]

C In = initial concentration in the apical (mg/L)