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Recombinant antimicrobial peptides efficiently produced using novel cloning and purification processes

After 30 min, a 100 L of acidic stop solution was added into each well and the absorbance at 450 nm was recorded using a using a Biotek Synergy Neo2 HTS Multi-Mode Microplate Reader (Mnnedorf, Switzerland)

Such was the case in this study, that the numbers of microglia in alcoholic brain were higher than those in mice given ALC plus alcohol or in the pair-fed controls