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10.1074/jbc.M112.433094 Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H: NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein

The usage of a rich medium (2xYT) in combination with a prolonged expression time (65 hours) at 20 C led to a substantial increase in jr PPO1-wt yield