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Upon subcutaneous injection, kisspeptin rapidly binds to kisspeptin receptors (GPR54) on GnRH neurons in the hypothalamus, causing immediate release of GnRH [7]

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To obtain the purified recombinant GHK peptide, the crude protein was incubated with a HisTrip HP His tag protein purification column (GE Inc., Marlborough, MA, USA) for 2 h in a 4 C room

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