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Gene 871:147437

After gently resuspending in annexinV binding buffer, the cells were incubated with annexinV-FITC/PI in the dark for 15 min and detected by flow cytometry using a BD FACSCantoll (Becton, Dickinson and Company, USA)

Blood was collected for complete blood count and serum biochemistry analyses

While clinical development for obesity was discontinued in 2007 after a Phase IIb trial failed to achieve its primary endpoint, the peptide remains a valuable research tool for investigating adipose tissue biology, lipolytic pathways, and receptor-independent signaling mechanisms

Chia ZJ, Cao YN, Little PJ, Kamato D