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After adding the water, do not shake the vial

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Techniques like Gel Permeation Chromatography (GPC) or rapid spin columns are often preferred over prolonged dialysis

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However, as age progresses, a decrease in GTP colocalization with mitochondria in old age indicates an age-related decline in GTP production by these enzymes and a possible shift to cytoplasmic NME1 and NME2 catalysis of ATP to GTP [46] localized near enlarged vesicles, as previously seen for Rab7 immunostaining [1] or invasive filopodia [47]