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The cells were left in the treatment medium for 24 h after which they were washed twice and collected for the luciferase assay, which was performed according manufacturers instructions with a kit from Promega Corporation (Madison, WI, USA)

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Plan your cycle length, breaks, and monitoring schedule with your clinician

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Sterile water contains no preservative, meaning bacteria can grow freely after the first needle puncture

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The circadian clock coordinates ribosome biogenesis

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*Correspondence: Vladimir Maletic, 107B Regency Commons Drive, Greer, SC 29650, USA e-mail: [email protected] This article was submitted to Affective Disorders and Psychosomatic Research, a section of the journal Frontiers in Psychiatry

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