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Pharmacological agents Experimental design 29 III) Cancer group: subcutaneously (s.c) inoculated with 2 x 10 4 MAT-LyLu cells only, IV) Diabetic + cancer (DC) group: inoculated with 2 x 10 4 MAT-LyLu cells and STZ injection, V) Cancer + metformin (CM) group: injected with metformin 30 for 14 days after Mat-LyLu cells application VI) Diabetic + cancer + metformin (DCM) group: metformin administered for 14 days together with STZ and Mat-LyLu cells

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demonstrated that D + Q was unable to eliminate senescent hepatocellular carcinoma (HCC) cells that had entered a state of senescence following prior doxorubicin treatment [116]

The morphological features of ferroptosis are quite specific, including a reduction (disappearance) of the mitochondrial cristae, rupture of the outer mitochondrial membrane, wrinkling, darkening of the mitochondria, iron-dependent nuclei without rupture, and cell membrane rupture