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Contribution of some other residues, namely, His401, Thr415, Thr417, E419, and Arg571, reported to be important for proper positioning of Gln398 and Thr399, was also assessed by the same group (Figure 3)

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Zhou SY, Gillilland M 3rd, Wu X, Leelasinjaroen P, Zhang G, Zhou H, Ye B, Lu Y, Owyang C

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The washout period forces the bodys endogenous NO synthase to resume its natural rhythm [3, 7]