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The A-side buffer contained 100 M Lucifer yellow dye, in transport buffer (1.98 g/L glucose in 10 mM HEPES, 1 Hank's balanced salt solution, pH 7.4), and the B-side buffer was transport buffer at pH 7.4

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Unfortunately, pain scores were not assessed at any earlier points in this study, it is therefore unknown whether pain had decreased more in the B12 group at an earlier time point