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The hierarchical heatmap was generated (Fig

10.1073/pnas.1705615114 30 DeyA.MaricC.KaesemeyerW

Take an appropriate amount of supernatant and add 10% formic acid in methanol-water (1:1, v/v) to dilute 20 times and vortex for 30 s

Third, we have shown that the acs1 mutant is hypersensitive to fluconazole in vitro and that deletion of ACS1 increases the efficacy of fluconazole during mammalian infection 23