glutathione s-transferase pull-down assays Pull down assay. Pull down assay was performed to study the interaction Purification of proteins fused to

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(2011): Found that glutathione deficiency is common in aging and metabolic syndrome, and supplementation with NAC can restore redox balance

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After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]

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