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Binding of Cu(II) ions to alpha-2 macroglobulin Our results demonstrated that 2M does not bind Cu(II) ions

HIS fusion proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen), and GST fusion proteins were purified with Pierce glutathione agarose (Thermo Scientific) according to the standard protocols provided by the manufacturers

Pyridostigmine should be started (oral tablets) at least several hours before exposure to soman (8 hours in advance is preferred) , and discontinued upon exposure to the nerve gas, at which point atropine and pralidoxime are given at the first indication of exposure (see video in Figure 3)

10.3390/jcm11030890 J Clin Med

4bd) were also significantly downregulated in Speg / compared with Speg +/+ hearts