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Effect of diphenyleneiodonium chloride on intracellular reactive oxygen species metabolism with emphasis on NADPH oxidase and mitochondria in two therapeutically relevant human cell types

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MitoSOX Red dye (4 M) or PI (1 g/mL) were added together with Hoechst nuclear counterstain (1 g/mL) (Bio-Sciences) to the cells in DPBS Ca 2+ /Mg 2+ and incubated for 20 minutes at 37 C protected from light