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The cell pellet was then resuspended in buffer A (supplemented with 1 mM DTT and 1 mM PMSF) for further purification or in KPi buffer if the CLs were needed for experiments

McCabea et al., 2007)

To monitor the transfer of the glutathione moiety from PfGrx to roGFP2, 5 M reduced roGFP2 WT , roGFP2 C151S or roGFP2 C208S were mixed with variable concentrations of glutathionylated PfGrx C88S or PfGrx C32S/C88S at room temperature for 1 h

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