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These findings suggest that the local conformational change at S309 regulates RIPK1 activation through a mechanism independent of its intrinsic kinase activity

The proteins were then transferred onto nitrocellulose membranes via Western blotting

Before staining, slides were thawed to room temperature for 30 min and then fixed in 10% neutral buffered formalin for 20 min, rinsed in double-distilled water, followed by rinsing in 60% isopropanol before being placed in working ORO-isopropanol stain (Rowley Biochemical, H-503-1B) for 5 min

This work was supported by the National Key Research and Development Programme of China (2022YFC2304700 to L.Z., and 2022YFC2304401 to L.Y.)

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