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Briefly, protein extracts were prepared by homogenizing with a Polytron homogenizer (KINEMATICA AG, Switzerland) in lysis buffer containing: 20 mM HEPES (pH 7.9), 25% v/v glycerol, 0.42 M NaCl, 0.2 mM EDTA, 1.5 mM MgCl2, 0.5% v/v Nonidet P-40, 1 mM DTT, 1 mM Naf, 1 mM Na 3 VO 4 , and 1 complete protease inhibitor cocktail (Roche Diagnostics Ltd, Mannheim, Germany)

Control received an equal volume of saline (5 ml/kg) immediately after strong alcohol, and presented minute gastric lesions, tongue affected, minute esophageal and duodenal lesions, as the redness, congestion as described (6, 10-13) but with intact mucosa

Data analysis after study completion showed a trend, and it is displayed in Fig

Recommended Botox units : 4 to 6 units

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