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Immunohistochemistry (IHC) To investigate hippocampal microglia, brain hemispheres were cryoprotected in a 30% sucrose solution prepared in 1 PBS for 24 h and subsequently embedded in Tissue-Tek optimal cutting temperature compound (Hartenstein Laborversand) at 70 C, following established protocols [121, 122]

Though the iCRRT cohort had longer intensive care unit (ICU) and hospital length of stay (LOS), there was no 30-, 60- or 90-day mortality in this group and 1-year post-LT mortality rates were comparable (Table 1)

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Likely because it's still an emerging field of research

10.3390/biom8030067 4 ArosioP.EliaL.PoliM