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188 However, long-term use of antibiotics can disrupt the gut microbiome or result in antimicrobial resistance complicating clinical treatment

The reaction was carried out in 10l reaction volume containing 25 ng of RNA, 0.25 M of each primer, 0.125 l iScript Reverse Transcriptase enzyme (BIO-RAD, Hercules, CA, USA), and 5 l of 2x one-step SYBR Green Reaction mix (BIO-RAD, Hercules, CA, USA)

and 1-cm is the length of the light path

All patients were followed up for at least 6 months

These metabolic changes may help boost the thorough health status of PTSD patients, lowering the risk of chronic inflammation (Rangel et al., 2025)