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The membranes were blocked for 1 h at room temperature in 5% non-fat dry milk (4259.01, SERVA, Heidelberg, Germany) prepared in Tris-buffered saline with Tween-20 (P7949, Sigma-Aldrich, Pozna, Poland) (TBST)

By keeping GH levels elevated through natural pulsatile release, Tesamorelin could protect against the muscle loss that rapid weight reduction often triggers

Carnitine acyltransferases: functional significance of subcellular distribution and membrane topology

The cells were cultured in basal medium supplemented with B27, 1 mM N-acetylcysteine, 10 nM gastrin, 50 ng/mL EGF, 1% R-spondin3 conditioned medium, 10 mM nicotinamide, 10 M Rho-kinase inhibitors, and 27 ng/mL Wnt surrogate