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H&E-stained sections were dehydrated with 75%, 90%, and 100% EtOH followed by 100% xylene and mounted with Eukitt mounting medium (Kindler GmbH)

Clinically, observational studies have linked specific microbiome unit reduced microbial diversity, depletion of butyrate-producing taxa, and an altered estrobolome to higher breast cancer risk or more aggressive phenotypes, and small interventional trials and pilot studies have explored probiotic supplementation (commonly Lactobacillus and Bifidobacterium strains) (176), showing encouraging signals such as improvement in systemic inflammatory markers, attenuation of gastrointestinal and mucosal toxicities during chemotherapy, mitigation of antibiotic-associated diarrhoea, modest improvements in metabolic parameters and bone health, and favourable changes in gut microbial composition

However, the IOM has set the UL for folic acid at 1.0 mg/day for adults [100, 108]