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The membrane was incubated with 5% (w/v) non-fat dried milk as a blocking solution and then probed with the indicated primary antibodies (Merck, USA), including anti-iNOS (1:2000) and anti--actin (1:2000)

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To elucidate the molecular origin of the 5-nitroimidazole ether activity switch, we solved high-resolution X-ray structures of monomeric HpTpx and in complex with both ligands (see Supplementary Section 1 for detailed structural information)

I2 and I3, two new EN460 analogs with different phenyl-substituted moieties, efficiently inhibited ERO1A, blunting VEGFA secretion