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For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT

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indicated that over half of the participants experienced full lesion healing and symptom abatement, with another 30% witnessing substantial lesion diminution and symptom relief

Long-term avoidance without addressing the underlying reasons for poor histamine clearance is neither sustainable nor necessary and risks contributing to the nutritional deficiencies that can worsen DAO function

The TEV protease and the -mercaptoethanol were added in the pooled fraction with target protein to TEV protease ratio of 1:10 (w/w) and the final concentration of 10 mM, respectively, to cleave 6HFh8 and dialyzed against 1 PBS with 150 mM NaCl at 4 C overnight