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[PubMed: 10569618] 143

qPCR (20 L) was carried out by using 2 Ultra SYBR Mixture (CWbio, China) in 12 8 well plates in a CFX Connect Real-Time System (Bio-Rad), containing 10 L of SYBR Mixture, 2 L cDNA, 1.6 L of qPCR primers (4 M), and 7.4 L nuclease-free water

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