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Several pharmacokinetic studies have investigated the improvement of the brain delivery extent by comparing the distribution of the released parent drug between the brain and plasma after prodrug dosing and parent drug administration in animal models (Table II)

The authors also acknowledge with thanks Science and Technology Unit, King Abdulaziz University for technical support

As published and reviewed elsewhere, AAV gene therapies, including liver-targeted gene-based editing approaches, have been developed to treat a wide variety of diseases, including rare diseases, such as hemophilia and spinal muscular atrophy (SMA) [40,41,42]

Key points The liver and gut are the main target organs for metformin

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis