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Hydroperoxides (ROOH) Hydroperoxides are unstable compounds formed via lipid (LOOH), protein (POOH), and DNA oxidation, originating primarily from three hepatic sources: (1) electron leakage from the mitochondrial electron transport chain (particularly Complex I/III), (2) ROS-generating activity of CYP2E1, and (3) the NOX enzyme family (predominantly NOX4)

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AgNPs accumulate to form hot spots, resulting in enhanced electromagnetic fields and thus enhanced infrared signals of glutathione

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Identifying such early metabolic alterations may ultimately guide the development of biomarkers for HAND risk stratification and enable timely intervention