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Sections were blocked in PBS with 10% donkey serum (DS) and 0.1% Triton X-100 for 2 h at room temperature, incubated with primary antibodies diluted in PBS with 3% donkey serum and 0.1% Triton X-100 overnight at 4 C and subsequently washed and incubated with secondary antibodies diluted in 0.05% BSA in PBS and DAPI for 2 h at room temperature

In this paper, measures to support natural detoxification pathways involving chelation, as well as use of pharmaceutical chelators are examined

10.1177/0960327119895811 67 LuH.WuL.LiuL.RuanQ.ZhangX.HongW.et al (2018)

4.2.5 Donor cell activity Similar to developmental maturation, activity-induced maturation can profoundly affect transplant integration

In this line, normal aging by itself appears to trigger astrocyte activation (Lin et al., 2007)