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Molecular Nutrition & Food Research, 59(7), 1364-1372

Transferred the mixture to an ultrafiltration centrifuge tube, centrifuged at 12,000 g for 20 min, discarded solution, added 50 mM NH 4 HCO 3 , centrifuged and discarded liquid, then added 50 mM NH 4 HCO 3 , centrifuged and discarded solution