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The relative miRNA expression of each gene was normalized to the expression of U6 RNA, while the relative mRNA expression of each gene was normalized to the expression of -actin mRNA

Biohacker Protocol (experimental) Dose: 300 mcg (1 capsule) Duration: 8 12 weeks Frequency: 1 capsule daily, continuous low-dose exposure Cycle Interval: 4 weeks off after each cycle Goal / Description: Explored for sustained mitochondrial biogenesis and metabolic adaptation in long-term models

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