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Guinea pigs were exposed to 27.03 mg/m(3) of VX or saline using a microinstillation inhalation exposure technique for 4 min and then the toxicity was assessed

Anal Chem 93(11):48944901 Oh C, Lee W, Park J et al (2023) Development of spleen targeting H(2)S donor loaded liposome for the effective systemic Immunomodulation and treatment of inflammatory bowel disease

Conservative protocol for beginners Goal: Assess tolerance and response before committing to full dosing Week 1: 50 mcg once daily (before bed, fasted) Week 2: 100 mcg once daily (before bed, fasted) Week 3-4: 100 mcg twice daily (morning and bedtime, both fasted) Week 5 onward: 100 mcg three times daily (if tolerated) This graduated approach allows researchers to assess their individual response to the appetite stimulation and any cortisol or prolactin-related effects before committing to full dosing

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W.OertelW.et al (2015)