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For deeper defects in flavin cofactor synthases such as FLAD1 and RFK, CRISPR/Cas9 correction and mRNA replacement have restored FAD production, mitochondrial membrane potential, and ROS control in patient-derived iPSC models, demonstrating translational promise (7274)

cerevisiae Saccharomyces cerevisiae strains used in this study are listed in Supplementary Table 1 and were cultured as previously described ( 600 0.61, were used in all experiments

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The primary antibodies were rabbit anti-FGF5 antibody (Thermo Fisher Scientific, USA), goat anti-SRD5A1 antibody (Abcam, UK), rabbit anti-androgen receptor antibody (Abcam, UK), goat anti-DKK1 antibody (Abcam, UK), beta-catenin polyclonal antibody (Proteintech, USA), c-MYC polyclonal antibody (Proteintech, USA), and beta tubulin monoclonal antibody (Proteintech, USA)

It is also widely used in dermatology as a treatment modality in depigmentation of hyperpigmented spots on the skin