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Though unable to provide atomic-level detail offered by X-ray crystallography or NMR, these methods were the first to reveal that many functional proteins exist as a dynamic ensemble of conformations rather than a single fixed structure

INGREDIENTS: Purified water

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Media was replaced every 2 days and monolayer integrity measured by TEER ( cm 2 , Millicell-ERS Voltohmmeter, Merck Millipore) every 7 days (16 h after media change)