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Stimulation was achieved by supplementing the serum-free tube-induction medium containing EBC-1 supernatant (50 g/ml), 3D culture supernatant derived from Lu99 cells described below or rhVEGF-A (30 ng/ml) with or without a mouse monoclonal anti-IgG2B antibody (10 g/ml) or an anti-VEGF-A neutralising antibody (10 g/ml) at the indicated time points

And then if Ive got the time doing another round to allow for the back and forth blood flow

If you believe the positive DSIP studies, then the conventional ~100 mcg is far below anything ever shown to do anything a single 5 mg vial would contain barely three studied doses

To evaluate differential expression (DE) attributable to hypoxia, HIF1 and HIF2 (EPAS1) knockdown, a trivariate model was applied using limma