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Ovarian patient-derived organoids (PDO) were maintained in ovarian media, which consisted of DMEM F12 media supplemented with the following components: 10 mM HEPES, 1X GlutaMax, 100 U/mL Penicillin/Streptomycin, 10 nM 17-B-Estradiol, 500 nM A83-01, 1X B27 without Vitamin A, 50 ng/mL EGF, 25 ng/mL FGF7, 100 ng/mL FGF10, 10 nM [Leu15]-Gastrin I, 10 ng/mL HGF, 20 ng/mL IGF, 1X N2, 1 mM N-Acetylcysteine, 10 ng/mL Neuregulin I, 10 mM Nicotinamide, 100 ng/mL Noggin, 100 g/mL Primocin, 10 nM Prostaglandin E2, 100 ng/mL R-Spondin 1, 3 M SB202190, and 10 M SB203580 (p38i)

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The overlapping targets between ZEA and male reproductive toxicity were identified using a bioinformatics platform ( Construction of Network and identification of Hub Genes The STRING database ( [15] was utilized to create a proteinprotein interaction (PPI) network, exporting general data in (tsv format) for the candidate targets