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Apolipoprotein (Apo) E is a protein central to lipid metabolism, neurobiology and neurodegenerative diseases

Human HEK 293 T (ATCC) cells were maintained in DMEM (Cellgro) supplemented with 10% fetal bovine serum (Sigma)

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The supernatant was dialyzed against potassium phosphate buffer (3 ml, 100 mm, pH 8.0) containing 0.05 mm PLP, 0.1 mm DTT and 0.1 mm EDTA for 24 h at 4C under dark conditions, which was used for enzyme assays

4.3 Cigarette smoke: the ferroptosis catalyst in COPD CS is the primary driver of COPD, acting as a potent catalyst for ferroptosis via multiple interconnected mechanisms