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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere

Impact on Laboratory Test Results When individuals receive methylcobalamin injections, changes in laboratory test outcomes such as serum B12 levels and homocysteine measurements may occur, which can impact the understanding of test results and possibly result in misdiagnosis or improper treatment adjustments by healthcare professionals analyzing lab work of patients undergoing methylcobalamin therapy

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As with all research peptides, the goal is to build a data set that can either justify more formal studies or reveal that the compound is not suitable for future development

Overall, the analysis of the three insomnia subtypes revealed that there were distinct differences in objective and subjective sleep variables between the subtypes, which are shown in Supplementary Table 3