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For proliferation assays, cells (5 10 4 per well) were plated in six-well plates with medium replacement every 48 h, followed by hemocytometer counting

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Its essential to be mindful of portion sizes and not rely solely on diet for skin whitening

At 48hours, a multiparametric assessment of the congestion is required

Differential analysis of plasma concentrations of metabolites from different categories revealed an increase in many phosphathidylcholines, triglycerides, and sphingomyelines in the plasma from cKOt mice (Figures 3J and Supplemental Figure 2B)