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Coverslips were then washed three times with PBS in the dark and then incubated in 500 L of 300 nM DAPI (Sigma-Aldrich, D9542) for 5 min at room temperature in the dark

IFFO1 interacted with the Rho-GTPase IQGAP3 through the 2 A coiled-coil domain To conduct the protein network of IFFO1 interactions in tumor cells, co-immunoprecipitation (co-IP) and mass spectrometry (IP-MS) analysis were performed using Flag-tagged IFFO1

An aggravated vata destroys the strength, complexion, happiness and creates stress in mind

Supplementary information Supplementary Figures 1-6, Supplementary Tables 1-3 and Supplementary References (PDF 2664 kb) Rights and permissions This work is licensed under a Creative Commons Attribution 4.0 International License

Many dermatologists also point out Most of the experiments carried so far, are only the preliminary observations taken from the pilot studies