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Amino acids and biogenic amines were quantified using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), while acylcarnitines, lipids and hexoses were analyzed by flow injection analysis mass spectrometry (FIA-MS/MS) on an ABI 4000 Q-Trap mass spectrometer (Applied Biosystems)

Expect 0.5-1 pound of fat loss per week at most, versus 1.5-3 pounds per week during an aggressive cut

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