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10.1038/nrurol.2017.69 101

One of the conjugates (N4Py-S-S-FA) contained a disulfide bond, which can be reduced intracellularly to facilitate release of N4Py

While the number of particles in the second reconstruction was much lower than the first, the data were consistent with a lower number of particles in a CT-like conformation (~ 17% of particles) relative to the bypass conformation (55% of particles) compared to the percentage of particles previously observed for the Amy-CTR-Gs complex (note, in the repeat experiment 28% of the particles could not be confidently classified, due to poorly resolved in ECD features) (Supplementary Fig