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After electrophoresis, the gel was rinsed with distilled water, and to confirm protein separation, it was incubated with Coomassie R-250 reagent for 30 min (1 g L 1 Coomassie R-250, 100 g L 1 glacial acetic acid, 40% (v/v) methanol)

On missing one pill, the missed Pill must be taken within 12 hours, even if it means having to take two pills at the same time on the next day

indicated that acetate can induce the disorder of cholesterol homeostasis by activating GPR43, thereby causing tubulointerstitial injury in DN (Hu et al., 2020)