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Reconstituted: Refrigerate at 28C and protect from light exposure

10.1016/j.cbpa.2021.02.009 53

The coverslips containing the cells were then washed with ice-cold PBS three times, fixed with 4% paraformaldehyde at 4 C for 30 min, permeabilized with Triton X-100 for 10 min, and subjected to three 5-min washes