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Analysis of Central Nervous System Karyopyknotic Cells Modified Bielschowskys silver staining and KlverBarrera staining (using KlverBarrera Luxol fast blue) were performed to demonstrate argentophilic neurites, axonal spheroids, and neuronal cell bodies, particularly in brain karyopyknotic areas ( (file:/ The brain was dissected according to NTP-7 at Levels 3 and 6 with neuroanatomic subsites presented in certain brain sections using coronal sections with three mandatory sections (Strbe et al., 2021) and analyzed using a semiquantitative neuropathological scoring system, as previously described (Strbe et al., 2021), the and combined score (08) = the sum of the analyzed affected areas (04) and karyopyknotic cells in the brain areas (04), as follows

This approach has demonstrated unique advantages in addressing complex diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases [8, 9]

Description Copper peptide GHK-Cu is a naturally occurring copper complex of the tripeptide glycyl-L-histidyl-L-lysine

The same volume of Cytotox-ONE Reagent (Promega, USA) was added into each well and the microplate was shaken for 30 secs to mix