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Pyruvate kinase M2 regulates hif-1alpha activity and IL-1beta induction and is a critical determinant of the warburg effect in LPS-activated macrophages

To prepare the K48/K29 tandemly-mixed Ub3 (K48/K29-Ub3 mix), K48-Ub2 (Proximal K11R K29R, Distal K11R K48R) was synthesized in the first reaction step (supplementary Fig