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For each set of analyses, the column was first conditioned with 100% mobile phase B (HPLC-grade acetonitrile), 1.2 ml/min for at least 5 min, and the temperature equilibrated at 25 C

J Biol Chem (2002) 277(30):2672932

How to implement: Dosing: 20-40 grams daily (post-workout or as snack) Choose undenatured whey (cold-processed, preserves bioactive peptides) Whey protein isolate (higher protein content, lower lactose) Best sources: Grass-fed whey isolate : Highest cysteine content Undenatured whey concentrate : Preserves immunoglobulins and glutathione precursors Avoid heat-treated : Heat denatures cysteine Timing: Post-workout (supports muscle recovery + glutathione synthesis) Morning (with breakfast) For vegans: Pea protein isolate : Lower in cysteine but can be paired with NAC Hemp protein : Moderate cysteine content Evidence: A randomized trial found that 20 grams of whey protein daily for 3 months increased lymphocyte glutathione by 35% and improved immune markers in HIV patients [15]

10.1016/j.dnarep.2009.08.003 167 JohnsonW

10.1186/s12890-024-02928-8 122