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The significance of differences in protein abundance was determined with a Paired T-test for sibling cohort comparisons and a T-test for comparisons of unrelated groups such as Stage 1 versus Stage 3

This overnight culture was then used to inoculate 100 mL of LB the next day and grown to an optical density ranging between 0.3 and 0.6

Images were collected using a CFI Plan Apochromat Lambda D oil immersion objective (60, NA = 1.4) and for roGFP imaging a CFI Plan Apo VC water immersion objective (60, NA = 1.2)

doi: 10.1691/ph.2019.8674 128 WangJNiZDuanZWangGLiF

It addresses one specific hormonal mechanism but does not stimulate follicles directly or address inflammation